Cuticular wax envelops the outermost layer of plant leaves, providing effective protection against various biotic and abiotic stresses. However, this protective barrier can be compromised by mechanical stress or herbivore attacks, and in some instances, it is deliberately breached during programmed organ shedding processes. While the signaling pathways involved in mechanical wounds and abscission have been extensively studied, the molecular mechanisms underlying the restoration of a damaged surface barrier remain largely unknown. In this study, we aimed to unravel the cellular reprogramming events that occur within internal tissues during the regeneration of the cuticle layer after abscission. To achieve this, we employed a combination of pharmacological approaches and forward and reverse genetic screenings. Through these methods, we successfully identified critical molecular regulators, including phytohormones and transcriptional networks, which play essential roles in the formation of the protective cuticle layer. We are currently engaged in characterizing the detailed pathway associated with these regulators. Additionally, to better understand the differences in the restoration process between programmed and accidental occurrences, we examined the healing process following a mechanical wound. These comparative analyses will provide a broader understanding of surface barrier restoration mechanisms.