Worldwide demand for vegetable oil is rapidly escalating due to increasing food, fuel and industrial requirements. Harnessing vegetative tissues for biofuel production has emerged as a promising avenue, particularly in high biomass crops. We have developed a high leaf oil platform represents a paradigm shift achieving oil accumulation levels akin to traditional oilseed crops within plant leaves. Through a strategic combination of gene overexpression and silencing with synergistic effect, we have achieved over 30% of triacylglycerol (TAG) content by dry weight in tobacco leaves. The approach enhances acyl editing within the eukaryotic pathway of glycerolipid assembly, while redirecting resources from the prokaryotic pathway, in high oil accumulating leaves. By translating the current success in tobacco leaf oil production into high biomass crops, we anticipate the oil yield per hectare exceeding traditional seed oil corps. This platform offers a compelling solution to meet increasing global demand for vegetable oil, marking game-changing sustainable energy and agricultural practices.