Plant metabolism comprises complex biochemical reactions that lead to the formation of various organic compounds playing important roles in plants. It can be divided into primary metabolism involved in functions essential for plant development and specialized (secondary) metabolism, which incorporates processes participating in adaptation and interactions with environment. Terpenes are one of the largest and most structurally diverse group of specialized metabolites that provide beneficial ecological, pharmacological, and economical effects. Terpenes are produced and stored in glandular trichomes, which are mainly multicellular epidermal structures found on most aerial plant tissues and characterized by the presence of secretory disc cells and storage cavity. Although terpene biosynthesis is well described in plants, little is known about their transcriptional and post-transcriptional regulation. In the current study, we used two glandular trichome producing plant species, cannabis and tomato, to investigate the transcriptional and post-transcriptional regulation of terpene biosynthesis via the identification of transcription factors (TFs) as key regulators. We applied complementary analyses of time series RNA-seq data including differential gene expression analysis. alternative splicing, weighted gene co-expression network analysis and gene regulatory network prediction to identify 10 and 15 putative candidate TFs for cannabis and tomato, respectively. We also show that some pathway genes targeted by the predicted TFs undergo alternative splicing. Functional characterisation of the candidate TFs is in progress.