Abstract
As the environment is changing day by day, plants have adapted mechanisms to adapt to the changing conditions. One such adaption is Post-translational modifications including Phosphorylation, Ubiquitination, SUMOylation, etc. Haloacid Dehalogenase phosphoprotein is a phosphatase involved in Inorganic phosphate starvation. Indeed, its role in dehydration stress is not been revealed. In this study, we have performed a genome-wide analysis to screen out different isoforms of HAD proteins present in Oryza sativa. A total of 13 OsHAD proteins were retrieved, identified, and characterized for their in-silico analysis including physiochemical properties, gene and protein structure, phylogenetic analysis, presence of cis-regulatory elements, gene ontology, etc. We also carried out transcriptome analysis to check out the expression level of found OsHAD genes. Lastly, we carried out OsHAD gene transformation in Chlamydomonas as well as Arabidopsis thaliana. Overall, our study provides an overview of the HAD proteins, their probable function and helps to reveal out their role in dehydration stress condition in Oryza sativa.