Canola (Brassica napus L.) is a yellow-flowering allopolyploid species in the family Brassicaceae. It is an important oilseed crop worldwide. Global warming has increased the frequency of heat stress at the reproductive stage of canola, a major factor limiting canola production. This study will evaluate the impact of heat stress on the reproductive process in canola. Microscopic observation accompanied by morphological and physiological measurements will help determine whether heat stress (32 °C/22 °C) at the reproductive stage differentially affects male and female microsporogenesis, gametogenesis, ovule fertilisation and/or early embryonic development. Genomics, transcriptomics and proteomics help identify heat-related genes and key regulatory pathways/networks, providing useful information for genetic improvement and exploring heat-tolerant canola crops.