The CRISPR/Cas13 system has been identified to be able to target single-stranded RNA for cleavage leading to gene silencing and for mRNA localisation, using an inactivated version of Cas13 fused with a fluorescent protein, in animals. The CRISPR/Cas13 system may also provide a useful tool to study plant biology, particularly for mRNA localization in tissues that are difficult to access such as pollen with its thick wall. Our goal was to assess the suitability of CRISPR/Cas13 system as a tool for plant biology. To test Cas13-mediated mRNA knock-down we designed a transient system in Nicotiana benthamiana (tobacco) targeting RFP, and stable transformation in Arabidopsis thaliana targeting the phytoene desaturase (PDS) gene, and tested two Cas13 proteins. Contrary to our expectations, one CRISPR/Cas13 system appeared to increase the expression of the target gene, while the other only provided a low level of knockdown. mRNA localization was tested in the transient system using a dead version of Cas13 with additional nuclear localization signals fused to GFP. However, we found that the Cas13 protein could exit the nucleus without a specific guide, and again saw an increase in target gene expression with the specific guide. We are now exploring the mechanism on how Cas13 increases expression of target genes.