A carnivorous plant, Drosera rotundifolia exhibits a remarkable ability to regenerate, generating adventitious shoots from detached leaves, suggesting induction of regeneration by wound stress. In this research, we established a tissue culture system to induce adventitious shoots from detached leaves, aiming to elucidate the regulatory mechanisms of how wounding stress regulates regeneration. Using stereo and metallurgical microscope, we observed adventitious shoot formation, confirming their development at the base of central tentacles. Additionally, we examined sections of shoot-forming leaves to investigate the origin and surrounding tissue structures of these shoots. Furthermore, we explored the effect of phytohormones, revealing that auxin and cytokinin enhance adventitious shoot formation. Now we are conducting a time course gene-expression analysis from leaf detachment to adventitious shoot formation. qRT-PCR analysis showed that the expression of DrWIND1 , whose homolog regulates wound-induced regeneration in Arabidopsis, was immediately upregulated after wounding suggesting its involvement in wound-induced regeneration in D.rotundifolia. We are also performing RNA-seq transcriptome analysis at the same timepoints. With these analyses, we aim to elucidate the molecular pathways regulating adventitious shoot formation, with a focus on the wound signaling pathway and phytohormone signaling pathways.