Albizia julibrissin is temperate tree species which is distributed throughout south and east Asia. The leaflets are sensitively closed up when touched or at night, the flowers are fragrant and attractive to bees so that they widely used for ornamental tree. Volatile triterpenoid and acylated steryl glycoside are representative bioactive compounds from flowers, bark, and roots for medicinal herbs of aging, carcinogenesis, and arteriosclerosis. Moreover, in vitro culture of multiple shoots was established by the medium supplementation effects of cytokinins in previous study. In our research, we investigated redifferentiation and biomass optimization rate derived from Albizia julibrissin compared with multiple shoots biomass accumulation and optimized culture condition in an air-lift bioreactor vessel. In vitro culture of multiple shoots were characterized with growth patterns for the ability to show uniform biomass amount (dry matter, growth index) for 6-weeks. The colorimetric absorbances of total phenolics content and total flavonoid content were supported mass-proliferated multiple shoots properties. Based on the biomass accumulation pattern, our shoot line described here enriched individual growth factors in whole cellular matrix depending on plant organs underwent redifferentiation or dedifferentiation as remaining cells, respectively. The assessment of optimal biomass accumulation of multiple shoots in this study could be obtain cosmeceutical and pharmaceutical metabolites by using sustainable biosynthesis platform. Moreover, it is possible to be protective way of ex situ conservation for Jeju native plant resources. The attractive Jeju native plant industrialization could be approached with the plant biotechnology to maximized the pilot-scale ingredient values with further studies.