Cell cycle regulation is a crucial step for the correct development of all organisms, and its progression depends on the activity of the Cyclin-Dependent Kinases (CDKs). CDK activity is regulated through association with regulatory subunits called Cyclins, by phosphorylation, dephosphorylation, interaction with inhibitory proteins, and proteolysis, mainly of the mitotic Cyclins. The ubiquitin-proteasome system (UPS) targets specific substrates, such as Cyclins, for degradation via proteasome 26S, which the Anaphase Promoting Complex/Cyclosome (APC/C) plays a crucial role in substrate recognition. In Arabidopsis thaliana, APC/C comprises 14 subunits, and alterations in their expression levels can alter plant growth and processes like gametogenesis. Cell division synchronizes with metabolic processes, with nutrient changes affecting metabolite concentrations and conversion rates, thereby modulating biological processes. Here, we aimed to functionally characterize A. thaliana plants overexpressing the APC5 gene. Two independent lines overexpressing APC5 were selected, and the results showed that the lines had a smaller rosette area, less leaves, smaller area of the first pair of leaves and a slower growth when compared to wild type plants. The lines also showed alterations in the expression levels in some cell cycle marker genes, like CYCD3;1 and CYCB1;1. However, metabolic differences in the primary metabolome weren't detected in the overexpression lines. In addition, gene promoter analysis showed specific expression of APC5 in the apical meristems and lateral root meristems, and subcellular localization of GFP-fused APC5 protein indicated predominant nuclear localization, with minor presence in the cytoplasm.