This study investigates the impact of Tuta absoluta infestation on the growth and physiology of five potato cultivars, aiming to identify susceptible and resistant cultivars. Tuta absoluta, a destructive insect pest of crops belonging to the Solanaceae family, has caused significant damage to tomato crops since its first recorded appearance in South Africa in 2016. The pest has also been observed to cause economic injury to potatoes.
The research methodology involves infesting the potato cultivars with L1 instar larvae of T. absoluta and assessing their growth and physiological responses during insect feeding. Various morphological parameters such as the number of leaves, number of mines, plant height, and damage severity will be recorded. Additionally, the trichome density of the cultivars will be measured using a scanning electron microscope to understand their potential role in resistance.
Gas exchange parameters including photosynthesis rate, transpiration rate, stomatal conductance, and intercellular carbon dioxide concentrations will be measured using a portable circuit and infrared gas analysis system (Li-6400, Li-Cor Inc., Lincoln, NE, USA). These measurements will provide insights into how T. absoluta infestation affects the physiological processes of the potato plants.
By examining both morphological and physiological responses, this study aims to close the knowledge gap regarding the impact of T. absoluta on different potato cultivars. The findings will contribute to understanding the varying susceptibility levels of potato cultivars to T. absoluta infestation, thereby facilitating the development of effective integrated pest management strategies for potato production.