In Brassicaceae vegetables, bolting can be pre-mature when unexpected low temperatures occur during cultivation, reducing food quality, such as flavour. Therefore, late bolting cultivars resistant to cold temperatures are desirable. However, strengthening the late bolting trait increases the period of cold treatment required for flowering, thereby hindering the efficiency of breeding new cultivars. Flowering Locus C (FLC) is a key regulator that suppresses bolting, and its gene expression is downregulated in response to low temperatures through an epigenetic mechanism. Here, we aim to adjust bolting time by targeting DNA methylation to the Brassica rapa (Chinese cabbage) FLC gene using the previously reported CRISPR/dCas9-mediated DNA methylation editing method (Papikian et al., 2019). We used complementation assays in a heterologous system in Arabidopsis thaliana to demonstrate delayed bolting by three BrFLC orthologues. Indeed, the BrFLC orthologues were functional, causing delayed flowering in A. thaliana. Furthermore, we investigated whether the targeted DNA methylation of BrFLC in A. thaliana can interfere with the epigenetic gene suppression mechanism in response to cold treatment. We anticipate that manipulation of DNA methylation can interfere with gene expression regulation also in B. rapa, thereby strengthening the late bolting trait even under low temperatures.